Skip to Main content Skip to Navigation
Journal articles

Quantitative Phosphoproteomic Analysis Reveals Shared and Specific Targets of Arabidopsis Mitogen-Activated Protein Kinases (MAPKs) MPK3, MPK4, and MPK6

Abstract : In Arabidopsis, mitogen-activated protein kinases MPK3, MPK4, and MPK6 constitute essential relays for a variety of functions including cell division, development and innate immunity. Although some substrates of MPK3, MPK4 and MPK6 have been identified, the picture is still far from complete. To identify substrates of these MAPKs likely involved in cell division, growth and development we compared the phosphoproteomes of wild-type and mpk3, mpk4, and mpk6. To study the function of these MAPKs in innate immunity, we analyzed their phosphoproteomes following microbe-associated molecular pattern (MAMP) treatment. Partially overlapping substrates were retrieved for all three MAPKs, showing target specificity to one, two or all three MAPKs in different biological processes. More precisely, our results illustrate the fact that the entity to be defined as a specific or a shared substrate for MAPKs is not a phosphoprotein but a particular (S/T) P phosphorylation site in a given protein. One hundred fifty-two peptides were identified to be differentially phosphorylated in response to MAMP treatment and/or when compared between genotypes and 70 of them could be classified as putative MAPK targets. Biochemical analysis of a number of putative MAPK substrates by phosphorylation and interaction assays confirmed the global phosphoproteome approach. Our study also expands the set of MAPK substrates to involve other protein kinases, including calcium-dependent (CDPK) and sugar nonfermenting (SnRK) protein kinases.
Document type :
Journal articles
Complete list of metadata

Cited literature [99 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-02083418
Contributor : Marianne Tardif Connect in order to contact the contributor
Submitted on : Wednesday, May 27, 2020 - 2:47:39 AM
Last modification on : Friday, October 15, 2021 - 5:25:15 PM

File

2018_Rayapuram_Molecular and C...
Publisher files allowed on an open archive

Licence


Copyright

Identifiers

Citation

Naganand Rayapuram, Jean Bigeard, Hanna Alhoraibi, Ludovic Bonhomme, Anne-Marie Hesse, et al.. Quantitative Phosphoproteomic Analysis Reveals Shared and Specific Targets of Arabidopsis Mitogen-Activated Protein Kinases (MAPKs) MPK3, MPK4, and MPK6. Molecular and Cellular Proteomics, American Society for Biochemistry and Molecular Biology, 2018, 17 (1), pp.61-80. ⟨10.1074/mcp.RA117.000135⟩. ⟨hal-02083418⟩

Share

Metrics

Record views

405

Files downloads

458