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Viral Metagenomics Approaches for High-Resolution Screening of Multiplexed Arthropod and Plant Viral Communities

Abstract : Viral metagenomic approaches have become essential for culture-independent and sequence-independent viral detection and characterization. This chapter describes an accurate and efficient approach to (1) concentrate viral particles from arthropods and plants, (2) remove contaminating non-encapsidated nucleic acids, (3) extract and amplify both viral DNA and RNA, and (4) analyze high-throughput sequencing (HTS) data by bioinformatics. Using this approach, up to 96 arthropod or plant samples can be multiplexed in a single HTS library.
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https://hal.archives-ouvertes.fr/hal-02154687
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Submitted on : Wednesday, June 12, 2019 - 8:55:15 PM
Last modification on : Sunday, November 29, 2020 - 8:12:01 PM

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Sarah François, Denis Filloux, Emmanuel Fernandez, Marie Helene Ogliastro, Philippe Roumagnac. Viral Metagenomics Approaches for High-Resolution Screening of Multiplexed Arthropod and Plant Viral Communities. Viral Metagenomics: Methods and Protocols, 1746, Humana Press Inc., 217 p., 2018, Methods in Molecular Biology, 978-1-4939-7683-6; 978-1-4939-7682-9. ⟨10.1007/978-1-4939-7683-6_7⟩. ⟨hal-02154687⟩

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