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Contribution of exofacial thiol groups in the reducing activity of Lactococcus lactis

Abstract : Lactococcus lactis can decrease the redox potential at pH 7 (E h7) from 200 to -200 mV in oxygen free Man-Rogosa-Sharpe media. Neither the consumption of oxidizing compounds or the release of reducing compounds during lactic acid fermentation were involved in the decrease in Eh7 by the bacteria. Thiol groups located on the bacterial cell surface appear to be the main components that are able to establish a greater exchange current between the Pt electrode and the bacteria. After the final Eh7 (-200 mV) was reached, only thiol-reactive reagents could restore the initial Eh7 value. Inhibition of the proton motive force showed no effect on maintaining the final Eh7 value. These results suggest that maintaining the exofacial thiol (-SH) groups in a reduced state does not depend on an active mechanism. Thiol groups appear to be displayed by membrane proteins or cell wall-bound proteins and may participate in protecting cells against oxidative stress.
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Déposant : Administrateur Agrosupdijon <>
Soumis le : vendredi 10 janvier 2020 - 08:26:53
Dernière modification le : dimanche 31 mai 2020 - 06:50:34



Damien Michelon, S. Abraham, Bruno Ebel, Joëlle De-Coninck, Florence Husson, et al.. Contribution of exofacial thiol groups in the reducing activity of Lactococcus lactis. FEBS Journal, Wiley, 2010, 277 (10), pp.2282-2290. ⟨10.1111/j.1742-4658.2010.07644.x⟩. ⟨hal-02434368⟩



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