Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

The identification of Plasmopara viticola genes potentially involved in pathogenesis on grapevine suggests new similarities between oomycetes and true fungi

Abstract : Plant diseases caused by fungi and oomycetes result in significant economic losses every year. Although phylogenetically distant, these organisms share many common features during infection. We identified genes in the oomycete Plasmopara viticola that are potentially involved in pathogenesis in grapevine by using fungal databases and degenerate primers. Fragments of P. viticola genes encoding NADH-ubiquinone oxidoreductase (PvNuo), laccase (PvLac), and invertase (PvInv) were obtained. PvNuo was overexpressed at 2 days postinoculation (dpi), during the development of the first hyphal structures and haustoria. PvLac was overexpressed at 5 dpi when genes related to pterostilbene biosynthesis were induced in grapevine. Transcript level for PvInv increased between 1 and 4 dpi before reaching a plateau. These results might suggest a finely tuned strategy of infection depending on nutrition and plant response. Phylogenetic analyses of PvNuo showed that P. viticola clustered with other oomycetes and was associated with brown algae and diatoms, forming a typical Straminipila clade. Based on the comparison of available sequences for laccases and invertases, the group formed by P. viticola and other oomycetes tended to be more closely related to Opisthokonta than to Straminipila. Convergent evolution or horizontal gene transfer could explain the presence of fungus-like genes in P. viticola.
Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal-univ-lyon1.archives-ouvertes.fr/hal-02522209
Déposant : Depot 1 Lyon 1 <>
Soumis le : vendredi 27 mars 2020 - 17:58:29
Dernière modification le : vendredi 26 février 2021 - 03:30:50

Lien texte intégral

Identifiants

Collections

Citation

P. Luis, A. Gauthier, S. Trouvelot, B. Poinssot, P. Frettinger. The identification of Plasmopara viticola genes potentially involved in pathogenesis on grapevine suggests new similarities between oomycetes and true fungi. Phytopathology, American Phytopathological Society, 2013, 103 (10), pp.130501115210007. ⟨10.1094/PHYTO-06-12-0121-R⟩. ⟨hal-02522209⟩

Partager

Métriques

Consultations de la notice

265