 |
Journal articles
Optimized assay and storage conditions for enzyme activity profiling of ectomycorrhizae
K. Pritsch
1
Pierre-Emmanuel Courty
2
J. L. Churin
2
Benoît Cloutier-Hurteau
3
M. Ali
3
Coralie Damon
4
Myriam Duchemin
3
S. Egli
5
J. Ernst
1
Laurence Fraissinet-Tachet
4
F. Kuhar
1
E. Legname
3
Roman Marmeisse
4, 2
A. Müller
5
P. Nikolova
1
M. Peter
5
Claude Plassard
3
F. Richard
6
M. Schloter
1
M. A. Selosse
6
Alain Franc
7
J. Garbaye
2
K. Pritsch 1
Author
Pierre-Emmanuel Courty 2
Author
J. L. Churin 2
Author
Benoît Cloutier-Hurteau 3
Author
Roman Marmeisse 4, 2
Author IdHAL : rolandmarmeisse ORCID : https://orcid.org/0000-0003-1653-3517
A. Müller 5
Author
P. Nikolova 1
Author
M. Peter 5
Author
Claude Plassard 3
Author Employer institution : Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement IdHAL : plassard-claude ORCID : https://orcid.org/0000-0002-5844-438X
F. Richard 6
Author
M. Schloter 1
Author
M. A. Selosse 6
Author
Alain Franc 7
Author
J. Garbaye 2
Author
1
German Research Center for Environmental Health - Helmholtz Center München (GmbH) (Ingolstädter Landstraße 1 · D-85764 Neuherberg - Germany)
2
IAM - Interactions Arbres-Microorganismes (INRA - Université de Lorraine, rue d'Amance, 54280 Champenoux - France)
- INRA - Institut National de la Recherche Agronomique : UMRA1136 (France)
- UL - Université de Lorraine (34 cours Léopold - CS 25233 - 54052 Nancy cedex - France)
3
UMR Eco&Sols - Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (place Viala, 34060 Montpellier, France - France)
- Montpellier SupAgro - Institut national d’études supérieures agronomiques de Montpellier (2, place Viala 34060 Montpellier Cédex 2 - France)
- Montpellier SupAgro - Centre international d'études supérieures en sciences agronomiques (2, place Pierre Viala - 34060 Montpellier Cedex 02 - France)
- Cirad - Centre de Coopération Internationale en Recherche Agronomique pour le Développement : UMR111 (Siège 42, rue Scheffer 75116 Paris - France)
- IRD - Institut de Recherche pour le Développement (Siège Le Sextant 44, bd de Dunkerque CS 90009 13572 Marseille cedex 02 - France)
- INRA - Institut National de la Recherche Agronomique : UMR1222 (France)
4
LEM - Laboratoire d'Ecologie Microbienne - UMR 5557 (BAT GREGOR MENDEL (ex 741)-4 et 43 Bvd du 11 Novembre 1918 69622 VILLEURBANNE CEDEX - France)
- INRA - Institut National de la Recherche Agronomique : UMR1418 (France)
- UCBL - Université Claude Bernard Lyon 1 (43, boulevard du 11 novembre 1918, 69622 Villeurbanne cedex - France)
- Université de Lyon (92 rue Pasteur - CS 30122, 69361 Lyon Cedex 07 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5557 (France)
- VAS - VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (Université de Lyon, VetAgro Sup, 69280 Marcy l'Etoile (campus vétérinaire); Université de Clermont, VetAgro Sup, 63370 Lempdes (campus agronomique) - France)
- ENVL - Ecole Nationale Vétérinaire de Lyon (France)
5
Swiss Federal Institute for Forest, Snow and Landscape Research WSL (Zürcherstrasse 111, CH-8903 Birmensdorf - Switzerland)
6
CEFE - Centre d’Ecologie Fonctionnelle et Evolutive (Centre National de la Recherche Scientifique - 1919 route de Mende - 34293 Montpellier CEDEX 5 - France)
- UPVM - Université Paul-Valéry - Montpellier 3 : UMR 5175 (Université Paul Valéry Montpellier 3 Route de Mende 34199 Montpellier Cedex 5 - France)
- EPHE - École pratique des hautes études : UMR 5175 (4-14 Rue Ferrus, 75014 Paris - France)
- PSL - Université Paris sciences et lettres (60 rue Mazarine 75006 Paris - France)
- UM - Université de Montpellier : UMR 5175 (163 rue Auguste Broussonnet - 34090 Montpellier - France)
- CNRS - Centre National de la Recherche Scientifique : UMR 5175 (France)
- IRD [France-Sud] - Institut de Recherche pour le Développement : UMR 5175 (911 avenue Agropolis, BP 64501 34394 Montpellier cedex 5 - France)
- INRAE - Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement : UMR 5175 (France)
- Institut Agro - Montpellier SupAgro (2, place Viala - Montpellier - France)
7
BioGeCo - Biodiversité, Gènes & Communautés (Site de recherche Forêt - Bois de Pierroton - 69, route d'Arcachon F-33612 Cestas Cedex FRANCE - France)
- INRA - Institut National de la Recherche Agronomique : UMR1202 (France)
- UB - Université de Bordeaux (35, place Pey Berland - 33076 Bordeaux - France)
Abstract : The aim of a joint effort by different research teams was to provide an improved procedure for enzyme activity profiling of field-sampled ectomycorrhizae, including recommendations on the best conditions and maximum duration for storage of ectomycorrhizal samples. A more simplified and efficient protocol compared to formerly published procedures was achieved by using manufactured 96-filter plates in combination with a vacuum manifold and by optimizing incubation times. Major improvements were achieved by performing the series of eight enzyme assays with a single series of root samples instead of two series, reducing the time needed for sample preparation, minimizing error-prone steps such as pipetting and morphotyping, and facilitating subsequent DNA analyses due to the reduced sequencing effort. The best preservation of samples proved to be storage in soil at 4\textendash6°C in the form of undisturbed soil cores containing roots. Enzyme activities were maintained for up to 4 weeks under these conditions. Short-term storage of washed roots and ectomycorrhizal tips overnight in water did not cause substantial changes in enzyme activity profiles. No optimal means for longer-term storage by freezing at -20°C or storage in 100% ethanol were recommended.
Keywords :
Biomedical and Life Sciences
Document type :
Journal articles
Domain :
Complete list of metadata
https://hal-univ-lyon1.archives-ouvertes.fr/hal-02540446
Contributor : Depot 1 Lyon 1 Connect in order to contact the contributor
Submitted on : Saturday, April 11, 2020 - 9:09:13 AM
Last modification on : Friday, May 20, 2022 - 9:04:09 AM
Contributor : Depot 1 Lyon 1 Connect in order to contact the contributor
Submitted on : Saturday, April 11, 2020 - 9:09:13 AM
Last modification on : Friday, May 20, 2022 - 9:04:09 AM
Links full text
Identifiers
- HAL Id : hal-02540446, version 1
- DOI : 10.1007/s00572-011-0364-4
- PRODINRA : 172575
- WOS : 000294812100001
Collections
UNIV-LYON1 | INRA | BIOENVIS | CNRS | UDL | INRAE | UNIV-MONTP3 | UNIV-LORRAINE | AGROPOLIS | CIRAD | UNIV-MONTPELLIER | EPHE | MONTPELLIER-SUPAGRO | IAM-UL | B3ESTE | CEFE | INRAEOCCITANIEMONTPELLIER | PSL | ECOMIC | A2F-UL | BIOGECO | ECOSOLS
Citation
K. Pritsch, Pierre-Emmanuel Courty, J. L. Churin, Benoît Cloutier-Hurteau, M. Ali, et al.. Optimized assay and storage conditions for enzyme activity profiling of ectomycorrhizae. Mycorrhiza, Springer Verlag, 2011, 21 (7), pp.589-600. ⟨10.1007/s00572-011-0364-4⟩. ⟨hal-02540446⟩
Metrics
Record views
Personal data
Terms and conditions