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Conference papers

Responses of pesticide impacted biofilms in a translocation experiment in river

Abstract : A translocation experiment was conducted in field within the Morcille river, a small French river draining a vineyard watershed (Beaujolais, 60km north of Lyon), to investigate the recovery of biofilms from pesticides. Biofilms were grown on artificial glass substrates glued on Plexiglas plates which were fixed to the riverbed both in a pristine (the reference) and in a pesticide impacted site of the river. Biofilms stemming from the latter one were transplanted to the pristine site after a colonization period of five weeks. The PICT (Pollution-Induced Community-Tolerance) concept was used to investigate the effects of pesticides on community composition and function and to monitor the recovery of the toxicant exposed community, once transplanted to the pristine site. Biofilms were collected at t0 (at translocation time), and one, three and five weeks after translocation both from the reference and the transplanted Plexiglas plates. The tolerance towards Diuron (a model herbicide for photosystem II inhibition and present in the Morcille river) was measured by photosynthesis short-term inhibition assays in laboratory to obtain dose-response curves and to calculate EC50 values. Structural changes of prokaryotic and eukaryotic communities inhabiting biofilms were examined by DGGE (Denaturing Gradient Gel Electrophoresis) of PCR-amplified 16S and 18S rRNA gene fragments. This study shows a 10-fold increase in tolerance (EC50 value) of the toxicant-exposed community when compared to the pristine community before translocation and a rapid recovery after translocation. DGGE analyses confirmed changes in the eukaryotic and prokaryotic community composition rather than changes in the total number of species, when comparing biofilms before translocation, pointing towards moderate and discontinuous contamination level in the downstream section of the Morcille river.
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Submitted on : Friday, May 15, 2020 - 2:05:07 PM
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  • HAL Id : hal-02590243, version 1
  • IRSTEA : PUB00023669



Ursula Dorigo, A. Berard, B. Motte, B. Volat, Agnès Bouchez, et al.. Responses of pesticide impacted biofilms in a translocation experiment in river. 5th SEFS, Palermo, ITA, 8-13 July 2007, 2007, pp.1. ⟨hal-02590243⟩



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