Skip to Main content Skip to Navigation
New interface
Journal articles

Multiscale quantification of morphodynamics: MorphoLeaf software for 2D shape analysis

Abstract : A major challenge in morphometrics is to analyse complex biological shapes formed by structures at different scales. Leaves exemplify this challenge as they combine differences in their overall shape with smaller shape variations at their margin leading to lobes or teeth. Current methods based on contour or on landmarks analysis are successful in quantifying either overall leaf shape or leaf margin dissection, but fail in combining the two. Here, we present a comprehensive strategy and its associated freely available platform for the quantitative, multiscale analysis of the morphology of leaves with different architectures. For this, biologically relevant landmarks are automatically extracted and hierarchized, and used to guide the reconstruction of accurate average contours that properly represent both global and local features. Using this method we established a quantitative framework of the developmental trajectory of Arabidopsis leaves of different ranks and retraced the origin of leaf heteroblasty. When applied to different mutant forms our method can contribute to a better comprehension of gene function as we show here for the role of CUC2 during Arabidopsis leaf serration. Finally, we illustrated the wider applicability of our tool by analysing hand morphometrics.
Complete list of metadata
Contributor : Migration ProdInra Connect in order to contact the contributor
Submitted on : Wednesday, May 27, 2020 - 10:14:05 AM
Last modification on : Monday, October 17, 2022 - 1:28:50 PM

Links full text



Eric Biot, Millan Cortizo Sabugo Cortizo, Jasmine Burguet, Annamaria Kiss, Mohamed Oughou, et al.. Multiscale quantification of morphodynamics: MorphoLeaf software for 2D shape analysis. Development (Cambridge, England), 2016, pp.3417-3428. ⟨10.1242/dev.134619⟩. ⟨hal-02632191⟩



Record views