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Characterization of enzymatically synthesized amylopectin analogs via asymmetrical flow field flow fractionation

Abstract : Asymmetrical flow field flow fractionation (AF4), when coupled with multi-angle laser light scattering (MALLS), is a very powerful technique for determination of the macromolecular structure of high molar mass (branched) polysaccharides. AF4 is a size fractionation technique just as size exclusion chromatography (SEC), nevertheless can overcome some crucial problems found in SEC analysis especially in starch like structures. This paper describes a detailed investigation of the macromolecular structure of two groups of well-defined synthetic amylopectin analogs - synthesized via an in vitro enzyme-catalyzed reaction using the enzymes phosphorylase b from rabbit muscle and Deinococcus geothermalis glycogen branching enzyme (Dg GBE). Size, molar mass distributions and structural data were studied by AF4 coupled with online quasi-elastic light scattering (QELS) and multi-angle light scattering (MALLS). (C) 2014 Elsevier Ltd. All rights reserved.
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Submitted on : Wednesday, May 27, 2020 - 10:25:44 PM
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Jelena Ciric, Agnès Rolland-Sabaté, Sophie Guilois, Katja Loos. Characterization of enzymatically synthesized amylopectin analogs via asymmetrical flow field flow fractionation. Polymer, Elsevier, 2014, 55 (24), pp.6271-6277. ⟨10.1016/j.polymer.2014.09.059⟩. ⟨hal-02637189⟩

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