Uncovering by Atomic Force Microscopy of an original circular structure at the yeast cell surface in response to heat shock - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Journal Articles BMC Biology Year : 2014

Uncovering by Atomic Force Microscopy of an original circular structure at the yeast cell surface in response to heat shock

Abstract

Background: Atomic Force Microscopy ( AFM) is a polyvalent tool that allows biological and mechanical studies of full living microorganisms, and therefore the comprehension of molecular mechanisms at the nanoscale level. By combining AFM with genetical and biochemical methods, we explored the biophysical response of the yeast Saccharomyces cerevisiae to a temperature stress from 30 degrees C to 42 degrees C during 1 h. Results: We report for the first time the formation of an unprecedented circular structure at the cell surface that takes its origin at a single punctuate source and propagates in a concentric manner to reach a diameter of 2-3 mu m at least, thus significantly greater than a bud scar. Concomitantly, the cell wall stiffness determined by the Young's Modulus of heat stressed cells increased two fold with a concurrent increase of chitin. This heat-induced circular structure was not found either in wsc1 Delta or bck1 Delta mutants that are defective in the CWI signaling pathway, nor in chs1 Delta, chs3 Delta and bni1 Delta mutant cells, reported to be deficient in the proper budding process. It was also abolished in the presence of latrunculin A, a toxin known to destabilize actin cytoskeleton. Conclusions: Our results suggest that this singular morphological event occurring at the cell surface is due to a dysfunction in the budding machinery caused by the heat shock and that this phenomenon is under the control of the CWI pathway.
Fichier principal
Vignette du fichier
2014_Pillet_BMC_1.pdf (1.93 Mo) Télécharger le fichier
Origin Publisher files allowed on an open archive
Loading...

Dates and versions

hal-02639992 , version 1 (28-05-2020)

Identifiers

Cite

Flavien Pillet, Stéphane Lemonier, Marion Schiavone, Cécile Formosa, Hélène Martin-Yken, et al.. Uncovering by Atomic Force Microscopy of an original circular structure at the yeast cell surface in response to heat shock. BMC Biology, 2014, 12 (6), 20p. ⟨10.1186/1741-7007-12-6⟩. ⟨hal-02639992⟩
87 View
53 Download

Altmetric

Share

More