Skip to Main content Skip to Navigation
Journal articles

Hydrolysis of concentrated raw starch: a new very efficient [alpha]-amylase from Anoxybacillus flavothermus

Abstract : A new [alpha]-amylase from Anoxybacillus flavothermus (AFA) was found to be effective in hydrolyzing raw starch in production of glucose syrup at temperatures below the starch gelatinization temperature. AFA is very efficient, leading to 77% hydrolysis of a 31% raw starch suspensions. The final hydrolysis degree is reached in 2-3 h at starch concentrations lower than 15% and 8-24 h at higher concentrations. AFA is also very efficient in hydrolyzing the crystalline domains in the starch granule. The major A-type crystalline structure is more rapidly degraded than amorphous domains in agreement with the observed preferential hydrolysis of amylopectin. Amylose-lipid complexes are degraded in a second step, yielding amylose fragments which then re-associate into B-type crystalline structures forming the final [alpha]-amylase resistant fraction. The mode of action of AFA and the factors limiting complete hydrolysis are discussed in details.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-02646168
Contributor : Migration ProdInra Connect in order to contact the contributor
Submitted on : Friday, May 29, 2020 - 4:07:32 AM
Last modification on : Saturday, June 25, 2022 - 9:23:18 PM

Identifiers

Collections

Citation

Georges Tawil, Anders Viksø-Nielsen, Agnès Rolland-Sabaté, Paul Colonna, Alain Buleon. Hydrolysis of concentrated raw starch: a new very efficient [alpha]-amylase from Anoxybacillus flavothermus. Carbohydrate Polymers, Elsevier, 2012, 87 (1), pp.46-52. ⟨10.1016/j.carbpol.2011.07.005⟩. ⟨hal-02646168⟩

Share

Metrics

Record views

8