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Effects of heat exposure on Akt/S6K1 signaling and expression of genes related to protein and energy metabolism in chicken (Gallus gallus) pectoralis major muscle

Abstract : In order to improve understanding of the heat-induced changes in muscle growth, we determined the expression of genes related to protein and energy metabolism in the pectoralis major muscle of chickens. We also explored the protein kinase B (PKB also called Akt)/p70 S6 kinase (S6K1)/S6 pathway that mediates anabolic signals thereby regulating metabolism and hypertrophic/atrophic balance. Four-week-old chickens were exposed to 32 or 22 degrees C for 1 week. Chickens from both groups were then fasted for 16 h or left fed, and submitted to an oral administration of glucose-arginine to induce an anabolic response (30-min treatment) or left untreated. High ambient temperature and the associated decrease in feed intake modified the expression of certain energy-related genes (e.g. -40% for PGC-1 alpha.) and protein metabolism (e.g. about +80% for atrogin-1), but the expression of several muscle metabolism-related genes considered here was unchanged. The capacity for muscle protein synthesis, i.e. RNA/protein ratio, was reduced in warm conditions (similar to-20%). Slightly lower activation of S6 induced by glucose-arginine treatment was found at 32 degrees C compared to 22 degrees C, which might indicate somewhat lower efficiency of mRNA translation. Analysis of glucose/insulin balance suggested changes in glucose metabolism under heat exposure. However, this remains to be characterized. (c) 2010 Elsevier Inc. All rights reserved.
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https://hal.inrae.fr/hal-02666115
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Submitted on : Sunday, May 31, 2020 - 8:02:49 AM
Last modification on : Monday, July 19, 2021 - 4:21:03 PM

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S. Boussaid-Om Ezzine, Nadia Everaert, Sonia Metayer-Coustard, Nicole Rideau, Cécile Berri, et al.. Effects of heat exposure on Akt/S6K1 signaling and expression of genes related to protein and energy metabolism in chicken (Gallus gallus) pectoralis major muscle. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, Elsevier, 2010, 157 (3), pp.281-287. ⟨10.1016/j.cbpb.2010.07.001⟩. ⟨hal-02666115⟩

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