Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88 - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Journal Articles (Review Article) Nature Biotechnology Year : 2007

Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88

Herman Pel (1) , Johannes de Winde (2, 1) , David Archer (3) , Paul Dyer (3) , Gerald Hofmann (4) , Peter Schaap (5) , Geoffrey Turner (6) , Ronald de Vries (7) , Richard Albang (8) , Kaj Albermann (8) , Mikael Andersen (4) , Jannick Bendtsen (9) , Jacques Benen (5) , Marco van den Berg (1) , Stefaan Breestraat (1) , Mark Caddick (10) , Roland Contreras (11, 12) , Michael Cornell (13) , Pedro Coutinho (14) , Etienne Danchin (14) , Alfons Debets (15) , Peter Dekker (1) , Piet van Dijck (1) , Alard van Dijk (1) , Lubbert Dijkhuizen (16) , Arnold Driessen (16) , Christophe D'Enfert (17, 18) , Steven Geysens (11) , Coenie Goosen (19) , Gert Groot (1) , Piet de Groot (20) , Thomas Guillemette (21) , Bernard Henrissat (14) , Marga Herweijer (1) , Johannes van den Hombergh (1) , Cees van den Hondel (22) , Rene van Der Heijden (23) , Rachel van Der Kaaij (19) , Frans Klis (20) , Harrie Kools (5) , Christian Kubicek (24) , Patricia van Kuyk (22) , Jürgen Lauber (25) , Xin Lu (24) , Marc van den Maarel (19) , Rogier Meulenberg (1) , Hildegard Menke (1) , Martin Mortimer (1) , Jens Nielsen (26) , Stephen Oliver (13) , Maurien Olsthoorn (1) , Karoly Pal (15, 27) , Noël van Peij (1) , Arthur Ram (22) , Ursula Rinas (28) , Johannes Roubos (1) , Cees Sagt (1) , Monika Schmoll (24) , Jibin Sun (28) , David Ussery (29) , János Varga (27, 30) , Wouter Vervecken (11, 12) , Peter van de Vondervoort (22) , Holger Wedler (25) , Han Wösten (7) , An-Ping Zeng (28) , Albert van Ooyen (1) , Jaap Visser (31) , Hein Stam (1)
Herman Pel
  • Function : Author
DSM
David Archer
  • Function : Author
Paul Dyer
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Jannick Bendtsen
  • Function : Author
Marco van den Berg
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DSM
Stefaan Breestraat
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DSM
Peter Dekker
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DSM
Piet van Dijck
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DSM
Alard van Dijk
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DSM
Gert Groot
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Marga Herweijer
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DSM
Johannes van den Hombergh
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DSM
Jürgen Lauber
  • Function : Author
Xin Lu
Rogier Meulenberg
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Hildegard Menke
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DSM
Martin Mortimer
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Jens Nielsen
Maurien Olsthoorn
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DSM
Noël van Peij
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DSM
Johannes Roubos
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DSM
Cees Sagt
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Holger Wedler
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Albert van Ooyen
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Hein Stam
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DSM

Abstract

The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid. We sequenced the 33.9-megabase genome of A. niger CBS 513.88, the ancestor of currently used enzyme production strains. A high level of synteny was observed with other aspergilli sequenced. Strong function predictions were made for 6,506 of the 14,165 open reading frames identified. A detailed description of the components of the protein secretion pathway was made and striking differences in the hydrolytic enzyme spectra of aspergilli were observed. A reconstructed metabolic network comprising 1,069 unique reactions illustrates the versatile metabolism of A. niger. Noteworthy is the large number of major facilitator superfamily transporters and fungal zinc binuclear cluster transcription factors, and the presence of putative gene clusters for fumonisin and ochratoxin A synthesis.

Dates and versions

hal-02668442 , version 1 (31-05-2020)

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Cite

Herman Pel, Johannes de Winde, David Archer, Paul Dyer, Gerald Hofmann, et al.. Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88. Nature Biotechnology, 2007, 25 (2), pp.221-231. ⟨10.1038/nbt1282⟩. ⟨hal-02668442⟩
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