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Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88

Herman Pel 1 Johannes de Winde 2, 1 David Archer 3 Paul Dyer 3 Gerald Hofmann 4 Peter Schaap 5 Geoffrey Turner 6 Ronald de Vries 7 Richard Albang 8 Kaj Albermann 8 Mikael Andersen 4 Jannick Bendtsen 9 Jacques Benen 5 Marco van den Berg 1 Stefaan Breestraat 1 Mark Caddick 10 Roland Contreras 11, 12 Michael Cornell 13 Pedro Coutinho 14 Etienne Danchin 14 Alfons Debets 15 Peter Dekker 1 Piet van Dijck 1 Alard van Dijk 1 Lubbert Dijkhuizen 16 Arnold Driessen 16 Christophe D'Enfert 17, 18 Steven Geysens 11 Coenie Goosen 19 Gert Groot 1 Piet de Groot 20 Thomas Guillemette 21 Bernard Henrissat 14 Marga Herweijer 1 Johannes van den Hombergh 1 Cees van den Hondel 22 Rene van Der Heijden 23 Rachel van Der Kaaij 19 Frans Klis 20 Harrie Kools 5 Christian Kubicek 24 Patricia van Kuyk 22 Jürgen Lauber 25 Xin Lu 24 Marc van den Maarel 19 Rogier Meulenberg 1 Hildegard Menke 1 Martin Mortimer 1 Jens Nielsen 26 Stephen Oliver 13 Maurien Olsthoorn 1 Karoly Pal 15, 27 Noël van Peij 1 Arthur Ram 22 Ursula Rinas 28 Johannes Roubos 1 Cees Sagt 1 Monika Schmoll 24 Jibin Sun 28 David Ussery 29 János Varga 27, 30 Wouter Vervecken 11, 12 Peter van de Vondervoort 22 Holger Wedler 25 Han Wösten 7 An-Ping Zeng 28 Albert van Ooyen 1 Jaap Visser 31 Hein Stam 1 
Abstract : The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid. We sequenced the 33.9-megabase genome of A. niger CBS 513.88, the ancestor of currently used enzyme production strains. A high level of synteny was observed with other aspergilli sequenced. Strong function predictions were made for 6,506 of the 14,165 open reading frames identified. A detailed description of the components of the protein secretion pathway was made and striking differences in the hydrolytic enzyme spectra of aspergilli were observed. A reconstructed metabolic network comprising 1,069 unique reactions illustrates the versatile metabolism of A. niger. Noteworthy is the large number of major facilitator superfamily transporters and fungal zinc binuclear cluster transcription factors, and the presence of putative gene clusters for fumonisin and ochratoxin A synthesis.
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Journal articles (Review article)
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https://hal.inrae.fr/hal-02668442
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Submitted on : Sunday, May 31, 2020 - 12:50:32 PM
Last modification on : Thursday, December 1, 2022 - 1:58:04 PM

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Herman Pel, Johannes de Winde, David Archer, Paul Dyer, Gerald Hofmann, et al.. Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88. Nature Biotechnology, 2007, 25 (2), pp.221-231. ⟨10.1038/nbt1282⟩. ⟨hal-02668442⟩

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