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Conference Papers Year : 2015

Improvement of hydrogen production from glycerol in microoxidative environment


Due to a current exponential growth of the biodiesel industry, the generation of glycerol waste as main byproduct, is expected to reach 5.8 billion pounds in 2020 (Ayoub et al, 2012). Bio-hydrogen is an advisable environmental friendly alternative to fossil fuels, and H2 production from glycerol by dark fermentation is a promising technology that is gaining a wide interest. The efficient conversion of bio-waste into H2 requires different active microbial populations where ecological as well as metabolic interactions between microorganisms could occur (Kajaste et al, 2014). The main microorganisms involved in hydrogen production belong to Clostridiaceae (strict anaerobes) and Enterobacteriaceae (facultative anaerobes) families. On the other hand, several bacterial species are able to consume H2 to produce acetate by homoacetogenesis. Using mixed cultures in a non-sterile environment, this study aims at operating a bioreactor under microoxidative conditions to promote the presence of hydrogen-producing microorganisms instead of strict anaerobic H2-consuming bacteria. Micro-oxidative environment were generated by adding hydrogenperoxide (H2O2) and the impact of the oxidizing agent concentration was evaluated on the microbial community dynamics and metabolisms. For this, H2O2 was added at a low constant flow rate (0.252 mL/min) at four different concentration (0.2, 0.4, 0.6 and 0.8% v/v) in a CSTR reactor fed with 15g/L glycerol, at a HRT of 12h, temperature of 37°C and regulated pH of 6.5. A mixture of aerobic and anaerobic sludge was used as inoculum. The H2 yields ranged between 0.1 and 0.47 mol H2.mol-1 glycerol. The effect of H2O2 concentration reached an optimal value at 0.6% with the highest hydrogen yield. It was concluded that the overall impact of H2O2 on the dark fermentation process resulted likely from the inhibition of H2 consumers at lower concentrations and a toxic effect on hydrogen-producing bacteria at higher concentrations.
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hal-02743128 , version 1 (03-06-2020)


  • HAL Id : hal-02743128 , version 1
  • PRODINRA : 305010


Florian Paillet, Fernando Silva Illanes, Antonella Marone, Estela Tapia Venegas, Léa Cabrol, et al.. Improvement of hydrogen production from glycerol in microoxidative environment. ICH2P-2015 - 6. International Conference on Hydrogen Production, University of Ontario Institute of Technology (UOIT). Oshawa, CAN., May 2015, Oshawa, Canada. pp.1048. ⟨hal-02743128⟩
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