C-Eci: A Cubic-Eci combined clearing method for 3D follicular content analysis In the ovary - Archive ouverte HAL Access content directly
Preprints, Working Papers, ... (Preprint) Year : 2020

C-Eci: A Cubic-Eci combined clearing method for 3D follicular content analysis In the ovary

(1) , (1) , (1) , (1) , (1) , (1) , (1) , (1)
1

Abstract

Deciphering mechanisms of oocyte development in female fishes still remains challenging and a comprehensive overview of this process at the level of the organ is still needed. The recent development optical tissue clearing methods have tremendously boosted the 3D imaging of large size biological samples that are naturally opaque. However, no attempt of clearing on fish ovary that accumulates extremely high concentration of lipids within oocytes has been reported to date. To face with this ovarian-specific challenge, we combined two existing clearing methods, the non-toxic solvent-based Eci method for efficient clearing and the CUBIC method to enhance lipid removal and reduce non-specific staining. The methyl green fluorescent dye was used to stain nuclei and delineate follicles. Using this procedure (named C-Eci), ovaries of both medaka and trout could be imaged in 3D and all follicles analyzed. To our knowledge this is the first procedure elaborated for clearing and imaging fish ovary in 3D. The C-Eci methods thus provides an interesting tool for getting precise quantitative data on follicular content in fish ovary and promises to be useful for further morphological studies.
Fichier principal
Vignette du fichier
2020_Lesage_BioRxiv_1.pdf (2.87 Mo) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

hal-02790859 , version 1 (05-06-2020)

Licence

Attribution - NonCommercial - CC BY 4.0

Identifiers

Cite

Manon Lesage, Manon Thomas, Jérôme Bugeon, Adèle Branthonne, Stéphanie Gay, et al.. C-Eci: A Cubic-Eci combined clearing method for 3D follicular content analysis In the ovary. 2020. ⟨hal-02790859⟩
59 View
53 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More