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Gene expression analysis in early embryos through reverse transcription quantitative PCR (RT-qPCR)

Nathalie Peynot 1 Véronique Duranthon 1 Daulat Raheem Khan 2
1 BDR - Biologie du Développement et Reproduction
2 Centre de Recherche en Biologie de la Reproduction (CRBR)
Abstract : Real-time, reverse transcription quantitative PCR (RT-qPCR) is a highly sensitive and reproducible technology for the analysis of gene expression patterns. Its ability to detect minute quantities of nucleic acid from multifarious sources makes it an ideal technique for embryonic transcript quantification. However, complex cellular diversity and active transcriptome dynamics in early embryos necessitate particular caution to avoid erroneous results. This chapter is intended to outline basic methodology to design and execute RT-qPCR experiments in pre-implantation embryos.
Keywords : embryo gene expression data analysis reference gene
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Submitted on : Friday, June 5, 2020 - 2:58:39 PM
Last modification on : Thursday, April 21, 2022 - 3:56:48 PM

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Nathalie Peynot, Véronique Duranthon, Daulat Raheem Khan. Gene expression analysis in early embryos through reverse transcription quantitative PCR (RT-qPCR). Nuclear reprogramming: Methods and Protocols, 1222 (2 ed.), Editions Springer, 290 p., 2015, Methods in Molecular Biology, 978-1-4939-1593-4. ⟨10.1007/978-1-4939-1594-1_14⟩. ⟨hal-02797641⟩

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