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Time-lapse imaging of developing meristems using confocal laser scanning microscope

Abstract : Analysis of shoot meristem shape and gene expression pattern has been conducted in many species over the past decades. Recent live imaging techniques have allowed an unprecedented accumulation of data on the biology of meristematic cells, as well as a better understanding of the molecular and biophysical mechanisms behind shape changes in this tissue. Here we describe in detail how to prepare shoot apices of both Arabidopsis and tomato, in order to image them over time using a confocal microscope equipped with a long-distance water-dipping lens.
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Submitted on : Friday, June 5, 2020 - 8:39:01 PM
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Olivier Hamant, Pradeep Das, Agata Burian. Time-lapse imaging of developing meristems using confocal laser scanning microscope. Plant cell morphogenesis: methods and protocols, 1080, Humana Press, 2014, Methods in Molecular Biology, 978-1-62703-643-6; 978-1-62703-642-9. ⟨10.1007/978-1-62703-643-6_9⟩. ⟨hal-02801293⟩



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