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NKP46 Marks caprine natural killer cells but is also present on some CD3 and TCR delta cells

Abstract : NKp46 has proven a reliable NK cell marker in both cattle and sheep. Four anti-bovine/ovine NKp46 mAbs reacted to caprine lymphocytes and specificity was confirmed as all mAbs reacted with caprine NKp46 transfectants. At young ages, goat NKp46+ cells were scarcer in circulation (~1% of PBMC) than in cattle and sheep, but the numbers increase with age. Similar to cattle and sheep, these cells are CD16+, CD8+, moderately perforin+, and CD2 marks a major negative and a minor positive subset. NKp46+ cells produce IFN-γ in response to cytokine stimulation, and following positive selection and culture they upregulate perforin and respond with NKp46-mediated cytotoxicity, thus confirming typical NK cell functions. NKp46 expression was non-overlapping with CD21+ B-cells, CD4+ T-cells and CD14+ monocytes. Most caprine-reactive gamma/delta TcR antibodies do not mark NKp46+ cells. However, in older goats, a significant proportion of NKp46+ cells express the TcR delta chain. This occurs at an age where the proportion of classical gamma-delta T-cells is in decline. NKp46+/TcRdelta+ cells were also present in cultures of IL-2 expanded caprine NKp46+ cells. Since NK cells in most species are CD3-/NKp46+, we stained permeabilized caprine PBMCs with a human cytoplasmatic CD3 epsilon mAb, which is cross-reactive in several species. Staining patterns corresponded well with expected T-cell populations, but this mAb also stained a subset of NKp46+ cells, the majority of which were also TcRdelta+. Thus, cytCD3e cannot be used straightforward for NK cell discrimination in goats, and these overlapping populations need closer investigation.
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Conference papers
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https://hal.inrae.fr/hal-02808691
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Submitted on : Saturday, June 6, 2020 - 5:46:22 AM
Last modification on : Friday, August 5, 2022 - 3:39:29 PM

Identifiers

  • HAL Id : hal-02808691, version 1
  • PRODINRA : 273881

Citation

Preben Boysen, Hege Lund, Fabrice Laurent, Timothy Connelley, Anne K Storset. NKP46 Marks caprine natural killer cells but is also present on some CD3 and TCR delta cells. 10. International Veterinary Immunology Symposium (IVIS), Istituto Zooprofilattico Sperimentale della Lombardia e dell' Emilia Romagna Bruno Ubertini. Brescia, ITA., Aug 2013, Milan, Italy. 254 p. ⟨hal-02808691⟩

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