LDL from obese patients with the metabolic syndrome show increased lipid peroxidation and activate platelets. - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Diabetologia Année : 2011

LDL from obese patients with the metabolic syndrome show increased lipid peroxidation and activate platelets.

Résumé

AIMS/HYPOTHESIS: This study assessed oxidative stress in LDL from obese patients with the metabolic syndrome and compared it with that in LDL from type 2 diabetic patients or control volunteers. It also determined the effect on platelets of LDL from the three groups. METHODS: The profiles of lipids, fatty acids and fatty acid oxidation products were determined in LDL isolated from plasma of patients with the metabolic syndrome, patients with type 2 diabetes and volunteers (n = 10 per group). The effects of LDL from the participant groups on the platelet arachidonic acid signalling cascade and aggregation were investigated. RESULTS: Compared with LDL from control volunteers, LDL from obese metabolic syndrome and type 2 diabetic patients had lower cholesteryl ester, higher triacylglycerol and lower ethanolamine plasmalogen levels. Proportions of linoleic acid were decreased in phosphatidylcholine and cholesteryl esters in LDL from both patient groups. Among the markers of lipid peroxidation, oxidation products of linoleic acid (hydroxy-octadecadienoic acids) and malondialdehyde were increased by 59% and twofold, respectively in LDL from metabolic syndrome and type 2 diabetic patients. LDL from metabolic syndrome and type 2 diabetic patients were equally potent in activating the platelet arachidonic acid signalling cascade through increased phosphorylation of p38 mitogen-activated protein kinase and cytosolic phospholipase A(2), and through increased thromboxane B(2) formation. LDL from patients with the metabolic syndrome and type 2 diabetes potentiated platelet aggregation by threefold and 3.5-fold respectively, whereas control LDL had no activating effects on platelets. CONCLUSIONS/INTERPRETATION: The metabolic syndrome in obese patients, without or with diabetes, is associated with increased oxidative stress in LDL, which triggers platelet activation.
Fichier principal
Vignette du fichier
Figure_3.pdf (46.5 Ko) Télécharger le fichier
Figure_1.pdf (10.78 Ko) Télécharger le fichier
Figure_2.pdf (93.94 Ko) Télécharger le fichier
Figure_4.PDF (46.38 Ko) Télécharger le fichier
MANUSCRIPT_Diabetologia_word.pdf (209.76 Ko) Télécharger le fichier
inserm-00616814_edited.pdf (347.89 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Origine : Fichiers produits par l'(les) auteur(s)
Origine : Fichiers produits par l'(les) auteur(s)
Origine : Fichiers produits par l'(les) auteur(s)
Origine : Fichiers produits par l'(les) auteur(s)
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

inserm-00616814 , version 1 (24-08-2011)

Identifiants

Citer

Romain Colas, Agnès Sassolas, Michel Guichardant, Christine Cugnet-Anceau, Myriam Moret, et al.. LDL from obese patients with the metabolic syndrome show increased lipid peroxidation and activate platelets.: Oxidative stress and metabolic syndrome. Diabetologia, 2011, 54 (11), pp.2931-40. ⟨10.1007/s00125-011-2272-8⟩. ⟨inserm-00616814⟩
311 Consultations
654 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More