Skip to Main content Skip to Navigation
Journal articles

Differential Use of the C-Type Lectins L-SIGN and DC-SIGN for Phlebovirus Endocytosis.

Abstract : Bunyaviruses represent a growing threat to humans and livestock globally. The receptors, cellular factors and endocytic pathways used by these emerging pathogens to infect cells remain largely unidentified and poorly characterized. DC-SIGN is a C-type lectin highly expressed on dermal dendritic cells that has been found to act as an authentic entry receptor for many phleboviruses (Bunyaviridae), including Rift Valley fever virus (RVFV), Toscana virus (TOSV) and Uukuniemi virus (UUKV). We found that these phleboviruses can exploit another C-type lectin, L-SIGN, for infection. L-SIGN shares 77% sequence homology with DC-SIGN and is expressed on liver sinusoidal endothelial cells. L-SIGN is required for UUKV binding but not for virus internalization. An endocytosis-defective mutant of L-SIGN was still able to mediate virus uptake and infection, indicating that L-SIGN acts as an attachment receptor for phleboviruses rather than an endocytic receptor. Our results point out a fundamental difference in the use of the C-type lectins L-SIGN and DC-SIGN by UUKV to enter cells, although both proteins are closely related in terms of molecular structure and biological function. This study sheds new light on the molecular mechanisms by which phleboviruses target the liver and also highlights the added complexity in virus-receptor interactions beyond attachment.
Document type :
Journal articles
Complete list of metadata

Cited literature [42 references]  Display  Hide  Download
Contributor : Michel Courcelles Connect in order to contact the contributor
Submitted on : Monday, April 23, 2018 - 11:41:22 AM
Last modification on : Tuesday, May 31, 2022 - 11:34:02 AM
Long-term archiving on: : Wednesday, September 19, 2018 - 1:11:46 AM


Distributed under a Creative Commons Attribution - NonCommercial - ShareAlike 4.0 International License



Psylvia Léger, Marilou Tetard, Berthe Youness, Nicole Cordes, Ronan N Rouxel, et al.. Differential Use of the C-Type Lectins L-SIGN and DC-SIGN for Phlebovirus Endocytosis.. Traffic, Wiley, 2016, 17 (6), pp.639-56. ⟨10.1111/tra.12393⟩. ⟨pasteur-01351418⟩



Record views


Files downloads