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Generating genomic platforms to study Candida albicans pathogenesis

Mélanie Legrand 1 Sophie Bachellier-Bassi 1 Keunsook Lee 2 Yogesh Chaudhari 2 Hélène Tournu 3 Laurence Arbogast 1 Hélène Boyer 1 Murielle Chauvel 1 Vitor Cabral 1 Corinne Maufrais 1, 4 Audrey Nesseir 1 Irena Maslanka 2 Emmanuelle Permal 1 Tristan Rossignol 1 Louise Walker 2 Ute Zeidler 1 Sadri Znaidi 1 Floris Schoeters 3 Charlotte Majgier 1 Renaud Julien 5 Laurence Ma 6 Magali Tichit 6 Christiane Bouchier 6 Patrick Van dijck 3 Carol Munro 2 Christophe D’enfert 1
1 BPF - Biologie et Pathogénicité fongiques
2 University of Aberdeen
3 KU Leuven - Catholic University of Leuven - Katholieke Universiteit Leuven
4 Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB
5 Modul-Bio
6 Biomics
CITECH - Centre de Recherche et Innovation Technologique
Abstract : The advent of the genomic era has made elucidating gene function on a large scale a pressing challenge. ORFeome collections, whereby almost all ORFs of a given species are cloned and can be subsequently leveraged in multiple functional genomic approaches, represent valuable resources toward this endeavor. Here we provide novel, genome-scale tools for the study of Candida albicans, a commen-sal yeast that is also responsible for frequent superficial and disseminated infections in humans. We have generated an ORFeome collection composed of 5099 ORFs cloned in a Gateway™ donor vector, representing 83% of the currently annotated coding sequences of C. albicans. Sequencing data of the cloned ORFs are available in the CandidaOrfDB database at http: //candidaorfeome.eu. We also engineered 49 expression vectors with a choice of promoters, tags and selection markers and demonstrated their applicability to the study of target ORFs transferred from the C. albicans ORFeome. In addition, the use of the OR-Feome in the detection of protein-protein interaction was demonstrated. Mating-compatible strains as well as Gateway™-compatible two-hybrid vectors were en
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Mélanie Legrand, Sophie Bachellier-Bassi, Keunsook Lee, Yogesh Chaudhari, Hélène Tournu, et al.. Generating genomic platforms to study Candida albicans pathogenesis. Nucleic Acids Research, Oxford University Press, 2018, 46 (14), pp.6935-6949. ⟨10.1093/nar/gky594⟩. ⟨pasteur-02015988⟩

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