Exploration of the role of the virulence factor ElrA during Enterococcus faecalis cell infection - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Scientific Reports Année : 2018

Exploration of the role of the virulence factor ElrA during Enterococcus faecalis cell infection

Yu Wei

Résumé

Enterococcus faecalis, an organism generally not pathogenic for healthy humans, has the potential to cause disease in susceptible hosts. While it seems to be equipped to interact with and circumvent host immune defense, most of the molecular and cellular mechanisms underlying the enterococcal infectious process remain elusive. Here, we investigated the role of the Enterococcal Leucine Rich protein A (ElrA), an internalin-like protein of E. faecalis also known as a virulence factor. ElrA was previously shown to prevent adhesion to macrophages. We show that ElrA does not inhibit the basic phagocytic process, but is able to prevent sensing and migration of macrophages toward E. faecalis. Presence or absence of FHL2, a eukaryotic partner of ElrA, does not affect the ElrA-dependent mechanism preventing macrophage migration. However, we highlight a partial contribution of FHL2 in ElrA-mediated virulence in vivo. Our results indicate that ElrA plays at least a dual role of which anti-phagocytic activity may contribute to dissemination of extracellular E. faecalis during infection.

Domaines

Bactériologie
Fichier principal
Vignette du fichier
s41598-018-20206-6.pdf (1.62 Mo) Télécharger le fichier
Loading...

Dates et versions

pasteur-02572353 , version 1 (13-05-2020)

Licence

Paternité

Identifiants

Citer

Natalia Nunez, Aurélie Derré-Bobillot, Stéphane Gaubert, Jean-Marie Herry, Julien Deschamps, et al.. Exploration of the role of the virulence factor ElrA during Enterococcus faecalis cell infection. Scientific Reports, 2018, 8 (1), pp.1749. ⟨10.1038/s41598-018-20206-6⟩. ⟨pasteur-02572353⟩
200 Consultations
60 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More