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Filtration biologique pour la réduction des éléments traces métalliques dans la biomasse du peuplier

Abstract : Phytostabilization is a gentle management option for sites polluted by trace elements (TE). Mycorrhizal fungi could assist plants in stabilizing pollutants by increasing the soil-prospected volume and by immobilizing MTE in their hyphae. Within the BIOFILTREE project, several approaches were used to select fungal strains that could be used for enhancing the phytostabilization process. Firstly, the mycorrhizal status of roots of three poplar clones from a TE-polluted site and the fungi associated with the roots were analyzed. The roots were colonized by endomycorrhizal, ectomycorrhizal, and endophytic fungi. Our data also revealed some specific trends, i.e. Hebeloma species was not associated with all poplar genotypes. Secondly, several fungal strains were isolated from polluted sites and their in vitro tolerance to TE was tested. There was a strong inter- and intra-specific variation in metal tolerance. In a greenhouse study, two poplar clones were inoculated with an endomycorrhizal inoculum and grown on a TE-polluted soil. A slight modification in TE accumulation in shoots was observed. In parallel, the role of ZIP (Zrt-Irt- like Proteins) and CDF (Cation Diffusion Facilitator) proteins in TE homeostasis/tolerance was also studied in the ectomycorrhizal model fungus Laccaria bicolor. The corresponding proteins were functionally characterized by the use of different approaches (yeast complementation, GFP-chimeric proteins, transcript analyses, cell metal content analyses). This study allowed us to better understand the mechanisms underlying zinc uptake and compartmentation in the hyphae of this fungus
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Submitted on : Thursday, March 29, 2018 - 12:29:56 PM
Last modification on : Wednesday, September 8, 2021 - 4:02:07 PM
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  • HAL Id : tel-01749854, version 1
  • PRODINRA : 277634



Laurence Didier Lacercat-Didier. Filtration biologique pour la réduction des éléments traces métalliques dans la biomasse du peuplier. Sylviculture, foresterie. Université de Lorraine, 2013. Français. ⟨NNT : 2013LORR0077⟩. ⟨tel-01749854⟩



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