Skip to Main content Skip to Navigation
New interface
Journal articles

Rabbit targeted genomic sequences after heterologous hybridization using human exome

Nathalie Iannuccelli 1, * Julien Sarry 1 Yvon Billon 2 Patrick Aymard 1 Virginie Helies 1 Cédric Cabau 1 Cécile Donnadieu 3 Julie Demars 1 
* Corresponding author
3 GeT-PlaGe - Génome et Transcriptome - Plateforme Génomique
GET - Plateforme Génome & Transcriptome, INRAE - Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement : US 1426
Abstract : Abstract Objective Causal mutations for major genes that underlie a broad range of morphological traits are often located within exons of genes that then affect protein functions. Non-model organism genetic studies are not easy to perform due to the lack of genome-wide molecular tools such as SNP genotyping array. Genotyping-By-Sequencing (GBS) methods offer an alternative. Consequently, we used this approach that is focused on the exome to target and identify major genes in rabbit populations. Data description We used a heterologous enrichment method before sequencing, allowing us to capture the rabbit exome using the marketed human panel since mammal protein coding genes are well conserved across the phylogenic tree of species. This targeted strategy was performed on 52 French rabbits from 5 different French strains (Californian, New-Zealand, Castor, Chinchilla and Laghmere). We generated 3.4 billion sequencing reads and approximately 29–140 million of reads per DNA sample. The expected exome coverage per sample ranged between 118 and 566X. The present dataset could be useful for the scientific community working on rabbit species in order to (i) improve the annotation of the rabbit reference genome Oryctolagus cuniculus (OryCun2.0), (ii) enrich the characterization of polymorphisms segregating in rabbits and (iii) evaluate the genetic biodiversity in different rabbit strains. Raw sequences were deposited in the European Nucleotide Archive (ENA) at the European Molecular Biology Laboratory- European Bioinformatics Institute (EMBL-EBI) data portal under bioproject accession number PRJEB37917.
Complete list of metadata

https://hal.inrae.fr/hal-03770192
Contributor : Nathalie SEGUIN Connect in order to contact the contributor
Submitted on : Tuesday, September 6, 2022 - 11:25:34 AM
Last modification on : Friday, October 28, 2022 - 2:09:11 PM

File

Lannuccelli-DN-2022.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Nathalie Iannuccelli, Julien Sarry, Yvon Billon, Patrick Aymard, Virginie Helies, et al.. Rabbit targeted genomic sequences after heterologous hybridization using human exome. BMC Research Notes, 2022, 15 (1), 3 p. ⟨10.1186/s13104-022-06162-5⟩. ⟨hal-03770192⟩

Share

Metrics

Record views

17

Files downloads

7