The ongoing biodiversity crisis is especially severe in freshwater habitats. Anthropized watersheds, such as the Seine-Normandie basin in France, are particularly affected by human interference. The study of fish species distribution in watersheds often relies on environmental drivers such as land use or climate. Yet, fish are also exposed to river connectivity constraints, such as dams, that are understudied despite their potential impact on fish dispersal. For this study, we investigated the role of local and whole-basin longitudinal connectivity in fish distribution. We designed connectivity indices based on river network characteristics and specific mobility for 33 species and included these indices in species distribution models, taking into account habitat suitability, to quantify their role in species distribution. Keeping the best index for each species, an average of 29 % – and up to 57 % – of explained fish distribution, depending on species, was tied to connectivity. We found that high con nectivity often had a significant and positive linear effect on species presence probability. Using a scoring system across multiple indices, we found connectivity indices that took local context into account (e.g. the ecological zonation of the river) performed consistently better than others. Indices that took only dispersal limitation into account scored higher for 12 species, while barriers, alone, were the most important constraint for 10 species, the remaining 11 being associated with both. This work points to fragmentation as a cause for lower likelihood of presence for many non-diadromous river fish species. It highlights the importance of considering both physical and functional connectivity constraints in fish distribution and provides additional insights for river management and restoration.