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Myomaker and Myomixer Characterization in Gilthead Sea Bream Under Different Myogenesis Conditions

Abstract : The skeletal muscle is formed by multinucleated myofibers originated by waves of hyperplasia and hypertrophy during myogenesis. Tissue damage triggers a regeneration process including new myogenesis and muscular remodeling. During myogenesis, the fusion of myoblasts is a key step that requires different genes’ expression, including the fusogens Myomaker and Myomixer. The present work aimed to characterize these proteins in gilthead sea bream and their possible role in in vitro myogenesis, at different fish ages and during muscle regeneration after induced tissue injury. Myomaker is a transmembrane protein highly conserved among vertebrates; whereas Myomixer is a micropeptide that is moderately conserved but maintains its crucial AxLyCxL motif. myomaker expression is restricted to skeletal muscle, while the expression of myomixer is more ubiquitous. In primary myocytes culture, myomaker and myomixer expression peaked at day 6 and day 8, respectively. During regeneration, the expression of both fusogens and all the myogenic regulatory factors showed a peak after 16 days post-injury. Moreover, myomaker and myomixer were present at different ages, but in fingerlings there were significantly higher transcript levels than in juveniles or adult fish. Overall, Myomaker and Myomixer are valuable markers of muscle growth that together with other regulatory molecules, can provide a deeper understanding of myogenesis regulation in fish.
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Preprints, Working Papers, ...
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https://hal.archives-ouvertes.fr/hal-03616921
Contributor : Jean-Charles Gabillard Connect in order to contact the contributor
Submitted on : Wednesday, March 23, 2022 - 9:30:37 AM
Last modification on : Thursday, March 31, 2022 - 3:41:23 AM
Long-term archiving on: : Friday, June 24, 2022 - 6:27:29 PM

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Miquel Perelló-Amorós, Aitor Otero-Tarrazón, Violeta Jorge-Pedraza, Isabel García-Pérez, Albert Sánchez-Moya, et al.. Myomaker and Myomixer Characterization in Gilthead Sea Bream Under Different Myogenesis Conditions. 2022. ⟨hal-03616921⟩

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