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Low occurrence of [i]Pseudomonas aeruginosa[/i] in agricultural soils with and without organic amendment

Abstract : The occurrence of Pseudomonas aeruginosa was monitored at a broad spatial scale in French agricultural soils, from various soil types and under various land uses to evaluate the ability of soil to be a natural habitat for that species. To appreciate the impact of agricultural practices on the potential dispersion of P aeruginosa, we further investigated the impact of organic amendment at experimental sites in France and Burkina Faso. A real-time quantitative PCR (qPCR) approach was used to analyze a set of 380 samples selected within the French RMQS ("Reseau de Mesures de la Qualite des Sols") soil library. In parallel, a culture-dependent approach was tested on a subset of samples. The results showed that P aeruginosa was very rarely detected suggesting a sporadic presence of this bacterium in soils from France and Burkina Faso, whatever the structural and physico-chemical characteristics or climate. When we analyzed the impact of organic amendment on the prevalence of P aeruginosa, we found that even if it was detectable in various manures (at levels from 10(3) to 10(5)CFU or DNA targets (g drywt)(-1) of sample), it was hardly ever detected in the corresponding soils, which raises questions about its survival. The only case reports were from a vineyard soil amended with a compost of mushroom manure in Burgundy, and a few samples from two fields amended with raw urban wastes in the sub-urban area of Ouagadougou, Burkina Faso. In these soils the levels of culturable cells were below 10 CFU (g drywt)(-1).
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Amelie Deredjian, Céline Colinon, Edmond Hien, Elisabeth Brothier, Benjamin Youenou, et al.. Low occurrence of [i]Pseudomonas aeruginosa[/i] in agricultural soils with and without organic amendment. Frontiers in Cellular and Infection Microbiology, 2014, 4, ⟨10.3389/fcimb.2014.00053⟩. ⟨hal-01192499⟩



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