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Complete genome sequence of a novel grapevine-infecting member of the genus Polerovirus, grapevine polerovirus 1

Abstract : Double-stranded RNAs and total RNAs purified from grapevine (Vitis vinifera) phloem scrapings of two varieties held in the INRAE (France) grapevine germplasm collection were analyzed by high-throughput sequencing. BLAST annota-tion revealed contigs with homology to Polerovirus genus members. The full genome sequence of one isolate (KT) was determined (5651 nucleotides [nt]), and a partial sequence representing about half of the genome was assembled for a second isolate (KS) that was found to share 95% nt sequence identity with the KT isolate. The genome has a typical polerovirus organization, containing six open reading frames (ORFs) as well as a putative additional ORF3a. Based on genome organization and phylogenetic relationships, the new virus belongs to the genus Polerovirus but, similar to the recently described persimmon polerovirus 1, is characterized by a highly divergent coat-protein/readthrough domain. Considering the species demarcation criteria for the family Luteoviridae, these two isolates, together with a closely related sequence recently deposited in the GenBank database (LC507098), represent a new Polerovirus species for which the name “Grapevine polerovirus 1” is proposed.
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https://hal.inrae.fr/hal-02565509
Contributor : Muriel Gauthier <>
Submitted on : Wednesday, May 6, 2020 - 3:01:25 PM
Last modification on : Wednesday, June 30, 2021 - 11:40:13 AM

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Thierry Candresse, Armelle Marais, Chantal Faure, Marie Lefebvre, Thierry Lacombe, et al.. Complete genome sequence of a novel grapevine-infecting member of the genus Polerovirus, grapevine polerovirus 1. Archives of Virology, Springer Verlag, 2020, 165 (7), pp.1683-1685. ⟨10.1007/s00705-020-04640-4⟩. ⟨hal-02565509⟩

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