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Suivi de la communauté microbienne d'un lisier de porc le long d'une filière d'élevage, approches moléculaire et culturale

Abstract : The microbial community evolution of a swine manure was monitored within a piggery equipped with a flushing system for a period of 6 months. Samples were withdrawn over all the manure management process : breeding house pits, homogenised storage pit, inlet and outlet of the compressor screw, storage lagoon, and finally soil before and after spreading of the manure from the lagoon. The microbial community was characterised and monitored using two different methodologies. The first one used techniques from molecular microbiology which allow the dominant microorganisms from manure to be identified and monitored without a need for culture (total bacteria, Archaea, Clostridiacea, Bacteroides Prevotella, and Lactobacillus-Streptococcus). The second, based on cultural techniques, was used to enumerate indicative bacteria, usually subdominant, as enterobacteria, enterococcus, coliforms, Escherichia coli, and potentially pathogenic bacteria as Salmonella, Listeria monocytogenes, and Staphylococcus aureus. Both approaches revealed a stability of the microbial populations during time within each step of the manure management process separately. However, qualitative and quantitative microbial population changes were observed when manure was shifting from one step of the process to another one (ex. going from the storage pit to the lagoon). Finally, microorganisms present within the effluent before spreading in the fields were not detectable anymore within soil after the spreading, whatever the technique used (molecular or cultural).
Mots-clés : TYPAGE MOLECULAIRE
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Conference papers
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https://hal.inrae.fr/hal-02588265
Contributor : Migration Irstea Publications <>
Submitted on : Friday, May 15, 2020 - 12:37:30 PM
Last modification on : Friday, February 12, 2021 - 3:33:53 AM

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  • HAL Id : hal-02588265, version 1
  • IRSTEA : PUB00020274

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P. Dabert, Hubert Brugère, Pascal Peu, Monique Kérourédan, A.M. Pourcher, et al.. Suivi de la communauté microbienne d'un lisier de porc le long d'une filière d'élevage, approches moléculaire et culturale. 36èmes Journées de la Recherche Porcine, Paris, 3-5 février 2004, 2004, pp.77-82. ⟨hal-02588265⟩

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