Skip to Main content Skip to Navigation
Journal articles

Applying the digital image correlation method to estimate the mechanical properties of bacterial biofilms subjected to a wall shear stress

Abstract : A digital image correlation (DIC) method was applied to characterize the mechanical behavior of Pseudomonas aeruginosa biofilms in response to wall shear stress using digital video micrographs taken from biofilm flow cells. The appearance of the biofilm in the transmitted light photomicrographs presented a natural texture which was highly conducive to random encoding for DIC. The displacement fields were calculated for two biofilm specimens. The DIC method concurred with previous analysis showing that biofilms exhibit viscoelastic behavior, but had the advantage over simple length measurements of longitudinal strain that it could precisely measure local strains in length (x) and width (y) within biofilm clusters with a 2 μm resolution as a function of time and wall shear stress. It was concluded that DIC was more accurate at measuring elastic moduli than simple length measurements, but that time-lapse 3D images would enable even more accurate estimates to be performed.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-02592631
Contributor : Migration Irstea Publications <>
Submitted on : Friday, May 15, 2020 - 4:26:14 PM
Last modification on : Tuesday, September 15, 2020 - 5:00:04 PM

Identifiers

  • HAL Id : hal-02592631, version 1
  • IRSTEA : PUB00027755

Collections

Citation

Jean-Denis Mathias, Paul Stoodley. Applying the digital image correlation method to estimate the mechanical properties of bacterial biofilms subjected to a wall shear stress. Biofouling, Taylor & Francis, 2009, 25 (8), pp.695-703. ⟨hal-02592631⟩

Share

Metrics

Record views

60