The effectiveness of solid waste aerobic biodegradation depends on the waste's initial biodegradable organic matter and on the active waste biomass content. Organic matter consists of three major components: i) polymeric substances, defined as the identifiable high-molecular-weight compounds such as carbohydrates and proteins; ii) simple substances, presented as sugars, amino acids and other small molecules; and iii) lignocellulosic compounds that are much more difficult to degrade. Only the first two components are readily metabolized by microorganisms as carbon and nitrogen sources.The aim of this work was, 1) to fractionate the organic matter of two different solid wastes: household waste residual (collected in exit sieve 50 mm) and sludge from agro-food wastewater treatment plant, 2) to develop new analytical methods (HPLC-SEC coupling with DEDL) in order to identify and quantify the carbohydrates substances in the different collected fractions, 3) to characterize the carbohydrates evolution in these soluble fractions during solid waste aerobic biodegradation kinetics, and 4) to determine the correlations between the chemical parameters and respirometry analysis in order to evaluate the biodegradable potential of solid waste.Coupling the characterization of the water soluble carbohydrates to the respirometric measurement led to propose an interpretation of the aerobic biodegradation processes of the waste organic matter. The beginning of the aerobic biodegradation seems to be correlated with the quantity of low-size carbohydrates molecules (DOM1