The presence of gastrointestinal helminths (GI helminths) was investigated in 725 murid rodents, trapped in various habitats of Nan, Loei and Buriram province in Thailand. The study revealed 17 species of rodents infected with 21 species or taxonomic groups of parasites (3 trematodes, 3 cestodes, 14 nematodes and 1 acanthocephalan). Overall prevalence of infection was 57.7% (418 infected out of 725 rodents). Among GI helminths, the dominant parasitic group was members of the family Trichostrongylidae (24.3%), followed by the cestodes Raillietina sp. (17.1%) and Hymenolepis diminuta (8.55%) and the nematode Syphacia muris (8.55%). The highest GI helminthic infection was found in Mus caroli (81.8%), followed by Mus cervicolor (76.5%), Leopoldamys edwardsi (75.0%), Bandicota indica (71.5%) and Bandicota savilei (71.4%). Highest rodent species richness (RSR) and helminth species richness (HSR) were found in Loei followed by Nan and Buriram. The helminth prevalence was higher in rodents from Nan followed by rodents from Loei and Buriram. Helminth infection among habitats showed that rodents from irrigated fields had highest infection followed by rodents from upland or dry agricultural area, forest and domestic habitat. The following parasites, Raillietina sp., Rodentolepis nana (syn. Hymenolepis nana), Hymenolepis diminuta, Moniliformis moniliformis and Cyclodontostomum purvisi, considered as potentially zoonotic parasites, were mainly found in rodents from domestic habitat and lowland irrigated fields.