Pulsed electric field treatment of apple tissues lead to a significant increase of juice yield by pressing [1]. The reason for this increase is not fully understood. NMR should help better understand the phenomena of de-structuring of the tissues by investigating the water compartmentalization into cells. In this context it was intended to carry out a screening of the impact of pulsed electric fields on the NMR relaxation times T2. Apple cylinders (1 cm in height and 0.5 cm in diameter) were examined by NMR (Minispec PC-120, 20 MHz, 20°C) after being subjected to a PEF treatment (500 to 1000 V / cm, 100 ms). The dry matter was determined by weighing before and after drying the cylinders examined by NMR. The measurements were made on three different apples for each modality. Control samples were taken from the same fruit. The NMR signals were fitted by a model comprising four relaxation components each of which was attributable to a fraction of water in the tissues [2, 3]. PEF treatment of 750 V / cm and 1000 V / cm altered the cellular structures. In particular, they acted on the vacuolar water, since the signal amplitude decreased in favour of cytoplasmic water and extracellular water. The decrease in relaxation time of water in apple parenchyma and the redistribution of vacuolar water to the cytoplasmic and extracellular compartments indicated a strong alteration of cell membranes leading to a redistribution of water and solutes between compartments. However, the T2 relaxation time of cytoplasmic water and extracellular water did not increase. This would indicate a release of molecules or ions in the aqueous phase that would help stiffen the cell wall.