During this study, nuclear magnetic resonance (NMR) was compared to the soxhlet extraction for determination of the lipid content of organic waste usually used in anaerobic digestion. Thus, 48 different wastes were analyzed using both methods and three replicates were performed for each. The correlation between Soxhlet methods and NMR was 0.90 and the regression slope was equal to 1.02. A better precision was obtained for NMR method with a coefficient of variation of 5%, while for the Soxhlet method, this coefficient is 14%. In contrast, the NMR method gave overall values below the reference method soxhlet. This difference can be explained by the advanced state of hydrolysis of fats where a large amount of volatile fatty acids, saturated molecules small chains linked would be present, and the degree of saturation of fatty acids long chains. NMR method seems suited to the analysis of lipids with better repetition and has the advantage of not using any solvent, be fast and non-destructive. However, the determination of some lipid matrices seems undervalued using the conditions established by the NMR protocol. Finally, this study also established data on lipid content of organic waste.