The success of conservation programs for genetically depleted species is challenging because survival and adaptive potential are thought to be promoted by genetic factors. Such programs are thus generally designed to minimize the risks associated with the loss of genetic diversity and inbreeding. This study suggests that a multi-year stocking strategy associated with sufficiently high numbers of unrelated parent pairs may be the best strategy for the successful reintroduction and preservation of genetic diversity for A. sturio. These findings are encouraging for the species and further highlights the relevance of careful genetic monitoring for the long-term conservation of genetically depleted species, which are more vulnerable to genetic effects.