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Article Dans Une Revue Biotechnology Advances Année : 2018

Synthetic biology tools for engineering Yarrowia lipolytica

Résumé

The non-conventional oleaginous yeast Yarrowia lipolytica shows great industrial promise. It naturally produces certain compounds of interest but can also artificially generate non-native metabolites, thanks to an engineering process made possible by the significant expansion of a dedicated genetic toolbox. In this review, we present recently developed synthetic biology tools that facilitate the manipulation of Y. lipolytica, including 1) DNA assembly techniques, 2) DNA parts for constructing expression cassettes, 3) genome-editing techniques, and 4) computational tools.

Dates et versions

hal-02626149 , version 1 (26-05-2020)

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Macarena Larroudé, Tristan Rossignol, Jean-Marc Nicaud, Rodrigo Ledesma Amaro. Synthetic biology tools for engineering Yarrowia lipolytica. Biotechnology Advances, 2018, 36 (8), pp.2150-2164. ⟨10.1016/j.biotechadv.2018.10.004⟩. ⟨hal-02626149⟩
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