Extensive field survey, laboratory and greenhouse studies reveal complex nature of <em>Pseudomonas Syringae-Associated</em> hazelnut decline in central italy - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2016

Extensive field survey, laboratory and greenhouse studies reveal complex nature of Pseudomonas Syringae-Associated hazelnut decline in central italy

Résumé

Pseudomonas avellanae (Pav) has been reported as the causal agent of bacterial decline and bacterial canker of hazelnut in Italy and Greece, respectively. Both hazelnut diseases were reported to be similar in terms of symptoms, severity and persistence. In this study, we found that both symptomatic and asymptomatic trees in the field were colonized by Pav. Multilocus Sequence Typing (MLST) analysis showed that Pav strains isolated during this study in Italy belong to the P. syringae phylogroup 1 and they are closely related to Pav strains previously isolated in Greece from hazelnut bacterial canker. On the other hand, strains isolated in earlier studies from hazelnut decline in Italy belong to both phylogroup 1 and 2 of P. syringae. Both phylogroup 1 strains of P. syringae from Greece and Italy are different than strains isolated in this study in terms of their capacity to excrete fluorescent pigments on different media. Despite the same plant genotype and cropping practices adopted, the incidence of hazelnut decline ranged from nearly 0 to 91% across our study sites. No disease developed on plants inoculated with Pav through wounding while leaf scar inoculations produced only mild disease symptoms. Based on our results and the previously reported correlation between pedo-climatic conditions and hazelnut decline, we conclude that hazelnut decline in central Italy could be incited by a combination of predisposing (adverse pedo-climatic conditions) and contributing factors (Pav). Because this is a true decline different from "bacterial canker" described in Greece, we refer to it as hazelnut decline (HD).
Fichier principal
Vignette du fichier
2016_Ram Lamichhane_Plos One_1.PDF (4.49 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02629555 , version 1 (27-05-2020)

Licence

Paternité

Identifiants

Citer

Jay Ram Lamichhane, Claudia Bartoli, Leonardo Varvaro. Extensive field survey, laboratory and greenhouse studies reveal complex nature of Pseudomonas Syringae-Associated hazelnut decline in central italy. PLoS ONE, 2016, 11 (2), pp.1-15. ⟨10.1371/journal.pone.0147584⟩. ⟨hal-02629555⟩
14 Consultations
36 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More