Identification, evolution, and expression partitioning of miRNAs in allopolyploid <em>Brassica napus</em> - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Article Dans Une Revue Journal of Experimental Botany Année : 2015

Identification, evolution, and expression partitioning of miRNAs in allopolyploid Brassica napus

Enhui Shen
  • Fonction : co premier-auteur
Ian Bancroft
  • Fonction : Auteur

Résumé

The recently published genome of Brassica napus offers for the first time the opportunity to gain insights into the genomic organization and the evolution of miRNAs in oilseed rape. In this study, 12 small RNA libraries from two B. napus cultivars (Tapidor and Ningyou7) and their four double-haploid lines were sequenced, employing the newly sequenced B. napus genome, together with genomes of its progenitors Brassica rapa and Brassica oleracea. A total of 645 miRNAs including 280 conserved and 365 novel miRNAs were identified. Comparative analysis revealed a high level of genomic conservation of MIRNAs (75.9%) between the subgenomes of B. napus and its two progenitors' genomes, and MIRNA lost/gain events (133) occurred in B. napus after its speciation. Furthermore, significant partitioning of miRNA expressions between the two subgenomes in B. napus was detected. The data of degradome sequencing, miRNA-mediated cleavage, and expression analyses support specific interactions between miRNAs and their targets in the modulation of diverse physiological processes in roots and leaves, as well as in biosynthesis of, for example, glucosinolates and lipids in oilseed rape. These data provide a first genome-wide view on the origin, evolution, and genomic organization of B. napus MIRNAs.

Dates et versions

hal-02636941 , version 1 (27-05-2020)

Identifiants

Citer

Enhui Shen, Jun Zou, Falk Hubertus Behrens, Li Chen, Chuyu Ye, et al.. Identification, evolution, and expression partitioning of miRNAs in allopolyploid Brassica napus. Journal of Experimental Botany, 2015, 66 (22), pp.7241-7253. ⟨10.1093/jxb/erv420⟩. ⟨hal-02636941⟩
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