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A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants

Abstract : Activated forms of jasmonic acid (JA) are central signals coordinating plant responses to stresses, yet tools to analyse their spatial and temporal distribution are lacking. Here we describe a JA perception biosensor termed Jas9-VENUS that allows the quantification of dynamic changes in JA distribution in response to stress with high spatiotemporal sensitivity. We show that Jas9-VENUS abundance is dependent on bioactive JA isoforms, the COI1 co-receptor, a functional Jas motif and proteasome activity. We demonstrate the utility of Jas9-VENUS to analyse responses to JA in planta at a cellular scale, both quantitatively and dynamically. This included using Jas9-VENUS to determine the cotyledon-to-root JA signal velocities on wounding, revealing two distinct phases of JA activity in the root. Our results demonstrate the value of developing quantitative sensors such as Jas9-VENUS to provide high-resolution spatiotemporal data about hormone distribution in response to plant abiotic and biotic stresses.
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Antoine Larrieu, Antony Champion, Jonathan Legrand, Julien Lavenus, David Mast, et al.. A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants. Nature Communications, Nature Publishing Group, 2015, 6, pp.1-8. ⟨10.1038/ncomms7043⟩. ⟨hal-02637267⟩

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