Skip to Main content Skip to Navigation
Journal articles

Noninvasive measurement of vulnerability to drought-induced embolism by x-ray microtomography

Abstract : Hydraulic failure induced by xylem embolism is one of the primary mechanisms of plant dieback during drought. However, many of the methods used to evaluate the vulnerability of different species to drought-induced embolism are indirect and invasive, increasing the possibility that measurement artifacts may occur. Here, we utilize x-ray computed microtomography (microCT) to directly visualize embolism formation in the xylem of living, intact plants with contrasting wood anatomy (Quercus robur, Populus tremula × Populus alba, and Pinus pinaster). These observations were compared with widely used centrifuge techniques that require destructive sampling. MicroCT imaging provided detailed spatial information regarding the dimensions and functional status of xylem conduits during dehydration. Vulnerability curves based on microCT observations of intact plants closely matched curves based on the centrifuge technique for species with short vessels (P. tremula × P. alba) or tracheids (P. pinaster). For ring porous Q. robur, the centrifuge technique significantly overestimated vulnerability to embolism, indicating that caution should be used when applying this technique to species with long vessels. These findings confirm that microCT can be used to assess the vulnerability to embolism on intact plants by direct visualization.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-02637984
Contributor : Migration Prodinra <>
Submitted on : Thursday, May 28, 2020 - 8:51:08 AM
Last modification on : Friday, July 9, 2021 - 12:02:04 PM

Links full text

Identifiers

Collections

Citation

Brendan Choat, Eric Badel, Régis Burlett, Sylvain Delzon, Hervé Cochard, et al.. Noninvasive measurement of vulnerability to drought-induced embolism by x-ray microtomography. Plant Physiology, American Society of Plant Biologists, 2016, 170 (1), pp.273-282. ⟨10.1104/pp.15.00732⟩. ⟨hal-02637984⟩

Share

Metrics

Record views

552