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Effects of nitrogen supply and of UV-C irradiation on the susceptibility of Lactuca sativa L to Botrytis cinerea and Sclerotinia minor

Abstract : Aims We tested here the effect of low nitrogen (N) availability and of UV-C radiations (a physical agent) on the susceptibility of Lactuca sativa L. towards Botrytis cinerea (BC 87) and Sclerotinia minor (SM). Methods We assessed first that a dose of UV-C radiation of 0.85 kJ. m−2 is effective in triggering a positive effect without entailing any negative effect on plants. We then tested the effect of this dose on 62-day-old lettuce plants grown at three N concentrations: 2 (low), 10 (normal) and 20 mM (high). Plants were inoculated 24 h after exposure to UV-C radiation and monitored for 4 days. Results Analysis of lesion areas showed that leaf susceptibility towards Botrytis cinerea (BC 87) and Sclerotinia minor (SM) substantially increased with the increasing N concentrations. Results also showed that the UV-C treatment can reduce MDA and H2O2 concentrations and lesion areas. Reduction of lesion areas reached 67 % on day 4 in the Sclerotinia minor (SM)-10 mM N trial and 52 % in the Botrytis cinerea (BC 87)-20 mM N trial. The UV-C treatment also resulted in an increase in the concentration of total phenolics in leaves which could be observed on days 1, 2 and 4 in Sclerotinia minor SM trial and days 1 and 2 in Botrytis cinerea BC 87 trial. Conclusions Taken together, our observations suggest that UV-C irradiation stimulate the capacity of lettuce plants to resist against Sclerotinia minor (SM) and Botrytis cinerea (BC 87) and that phenolic compounds may be involved in this resistance.
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https://hal.inrae.fr/hal-02638136
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Submitted on : Thursday, May 28, 2020 - 9:36:03 AM
Last modification on : Friday, February 5, 2021 - 4:38:01 PM

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Chayma Ouhibi, Houneida Attia, Philippe Nicot, François Lecompte, Véronique Vidal, et al.. Effects of nitrogen supply and of UV-C irradiation on the susceptibility of Lactuca sativa L to Botrytis cinerea and Sclerotinia minor. Plant and Soil, Springer Verlag, 2015, 393 (1-2), pp.35-46. ⟨10.1007/s11104-015-2458-9⟩. ⟨hal-02638136⟩

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