Eleven polymorphic microsatellite markers were developed from enriched DNA libraries for the invasive Western conifer seed bug, Leptoglossus occidentalis. The number of alleles ranged from 2 to 11 and observed heterozygosities from 0.038 to 0.933. Additional results of cross-species amplifications are reported for two congeneric species. This set of microsatellite markers, the first one available for L. occidentalis, enables further investigations of population structure of this species which represents a serious threat for European conifer regeneration.