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Limited elimination of two viruses by cryotherapy of Pelargonium apices related to virus distribution

Abstract : The possibility of eradicating the pelargonium flower break virus (PFBV) and pelargonium line pattern virus (PLPV) by cryotherapy of axillary shoot apices was investigated using five Pelargonium cultivars. Viruses were detected by DAS-ELISA and their location was determined by immunolocalization. Apex culture did not permit elimination of PFBV and only 15% regenerated plants of 'Stellar Artie' cultivar were ELISA PLPV-negative. Plants regenerated from cryotherapy-treated apices were tested by DAS-ELISA after a 3-month in vitro culture period. Viruses were not detected in 25% and 50% of the plants tested for PFBV and PLPV, respectively. However, immunolocalization carried out on apices originating from cryopreserved shoot tips sampled from DAS-ELISA negative plants showed that they were still virus-infected. Using immunolocalization, PFBV and PLPV could be detected in Pelargoniuni apices, even in the meristematic dome. However, viral particles were more numerous in basal zone cells than in meristematic cells. Our results demonstrate that PFBV and PLPV are present within meristematic cells and that cryopreservation can partly reduce the quantity of these viruses in Pelargonium plants but not eliminate them totally. Additional knowledge on localization and behaviour of viruses during cryopreservation is essential to optimize cryotherapy and plant genetic resource management.
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Submitted on : Thursday, May 28, 2020 - 6:27:34 PM
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  • HAL Id : hal-02642556, version 1
  • PRODINRA : 47042
  • WOS : 000290888700003


Anthony Gaillard, R. Mallet, Michel Chevalier, Agnès Grapin. Limited elimination of two viruses by cryotherapy of Pelargonium apices related to virus distribution. Cryo Letters, Cryo Letters, 2011, 32 (2), pp.111-122. ⟨hal-02642556⟩



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