In this paper, we attempt to ascertain to what extent the clusters identified in the agricultural and agri-food space rely on a spatial dynamic involving real agricultural and agri-food activities in the relevant geographic area. We use explanatory spatial data analysis (ESDA) to detect the spatial structure and dynamics of agri-food activities and to connect them to the competitiveness clusters’ locations. Results show that the six clusters specifically studied have different profiles because of their proximity to dynamic areas of agricultural and agrifood production and because of their collaborations with other clusters.