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Functional characterization of the HD-ZIP IV transcription factor OCL1 from maize

Abstract : OCL1 (OUTER CELL LAYER1) encodes a maize HD-ZIP class IV transcription factor (TF) characterized by the presence of a homeo DNA-binding domain (HD), a dimerization leucine zipper domain (ZIP), and a steroidogenic acute regulatory protein (StAR)-related lipid transfer domain (START) involved in lipid transport in animals but the function of which is still unknown in plants. By combining yeast and plant trans-activation assays, the transcriptional activation domain of OCL1 was localized to 85 amino acids in the N-terminal part of the START domain. Full-length OCL1 devoid of this activation domain is unable to trans-activate a reporter gene under the control of a minimal promoter fused to six repeats of the L1 box, a cis-element present in target genes of HD-ZIP IV TFs in Arabidopsis. In addition, ectopic expression of OCL1 leads to pleiotropic phenotypic aberrations in transgenic maize plants, the most conspicuous one being a strong delay in flowering time which is correlated with the misexpression of molecular markers for floral transition such as ZMM4 (Zea Mays MADS-box4) or DLF1 (DELAYED FLOWERING1). As suggested by the interaction in planta between OCL1 and SWI3C1, a bona fide subunit of the SWI/SNF complex, OCL1 may modulate transcriptional activity of its target genes by interaction with a chromatin remodelling complex.
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https://hal.inrae.fr/hal-02645440
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Submitted on : Friday, May 29, 2020 - 1:41:26 AM
Last modification on : Monday, May 10, 2021 - 2:58:19 PM

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Nathalie Depege-Fargeix, Marie Javelle, Pierre Chambrier, Nathalie Frangne, Denise Gerentes, et al.. Functional characterization of the HD-ZIP IV transcription factor OCL1 from maize. Journal of Experimental Botany, Oxford University Press (OUP), 2011, 62 (1), pp.293-305. ⟨10.1093/jxb/erq267⟩. ⟨hal-02645440⟩

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