The interest in probiotics as remedies for a broad number of gastrointestinal and other infectious diseases has gained wide interest over the last years. The goal of this study is the investigation of the probiotic potential of a human isolate of Lactobacillus paracasei subsp. paracasei. Its resistance to simulated gastrointestinal conditions and adhesion to Caco-2 cells were examined and its influence on the growth of the enteropathogenic Escherichia coli (EPEC) strain E2348/69 and Salmonella Typhimurium LT2 in mixed cultures was investigated. The strain was unaffected by pH 3, pancreatin or bile salts, and was able to survive at pH 2 in the presence of pepsin. A strong inhibition of EPEC and Salmonella populations was observed within 24 h, indicating a high antagonistic activity of the strain towards the two enteropathogens. In addition, the strain was able to adhere to Caco-2 cells and to inhibit the adhesion of E. coli E2348/69 and Salmonella Typhimurium LT2 using either pre-incubation or co-incubation procedures. This strain was therefore found, in vitro, to possess desirable probiotic properties. Moreover, the growth of five potential pathogens (Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus cereus, Staphylococcus aureus and Enterococcus faecalis) was found to be inhibited