A-type single crystals were prepared in dilute water/acetone solutions by crystallizing narrow fractions of short amylose chains biosynthesized in vitro. The 5 mu m-long spindle-shaped crystals were observed by scanning and transmission electron microscopy. Electron diffraction patterns were recorded from frozen-hydrated crystals at different tilt angles around selected crystallographic axes. In particular, a series of patterns corresponding to [hkO] zone axes was collected by rotation around the long dimension of the crystal. A description of the crystal habit was proposed by correlating the electron diffraction data with the microscopy images. The double helices are oriented along the long dimension of the crystal (c-axis) and packed in a parallel fashion into lamellae with a parallelogram cross-section defined by the a and b axes of the monoclinic unit cell. A-amylose single crystals are polar objects that grow in the direction of the reducing end of the amylose chains, and the c-axis is oriented opposite to the growth direction. (C) 2011 Elsevier Ltd. All rights reserved.