Skip to Main content Skip to Navigation
Journal articles

Glutathione transferases of Phanerochaete chrysosporium S-glutathionyl-p-hydroquinone reductase belongs to a new structural class

Abstract : The white rot fungus Phanerochaete chrysosporium, a saprophytic basidiomycete, possesses a large number of cytosolic glutathione transferases, eight of them showing similarity to the Omega class. PcGSTO1 (subclass I, the bacterial homologs of which were recently proposed, based on their enzymatic function, to constitute a new class of glutathione transferase named S-glutathionyl-(chloro)hydroquinone reductases) and PcGSTO3 (subclass II related to mammalian homologs) have been investigated in this study. Biochemical investigations demonstrate that both enzymes are able to catalyze deglutathionylation reactions thanks to the presence of a catalytic cysteinyl residue. This reaction leads to the formation of a disulfide bridge between the conserved cysteine and the removed glutathione from their substrate. The substrate specificity of each isoform differs. In particular PcGSTO1, in contrast to PcGSTO3, was found to catalyze deglutathionylation of S-glutathionyl-p-hydroquinone substrates. The three-dimensional structure of PcGSTO1 presented here confirms the hypothesis that it belongs not only to a new biological class but also to a new structural class that we propose to name GST xi. Indeed, it shows specific features, the most striking ones being a new dimerization mode and a catalytic site that is buried due to the presence of long loops and that contains the catalytic cysteine.
Document type :
Journal articles
Complete list of metadata

Cited literature [62 references]  Display  Hide  Download

https://hal.inrae.fr/hal-02648116
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Friday, May 29, 2020 - 8:52:08 AM
Last modification on : Wednesday, September 8, 2021 - 4:02:08 PM

File

2011_Meux_Journal of Biologica...
Publisher files allowed on an open archive

Licence


Copyright

Identifiers

Citation

Edgar Meux, Pascalita Prosper, Andrew Ngadin, Claude Didierjean, Mélanie Morel, et al.. Glutathione transferases of Phanerochaete chrysosporium S-glutathionyl-p-hydroquinone reductase belongs to a new structural class. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2011, 286 (11), pp.9162-9173. ⟨10.1074/jbc.M110.194548⟩. ⟨hal-02648116⟩

Share

Metrics

Record views

45

Files downloads

56