Skip to Main content Skip to Navigation
Journal articles

Two-electron reduction of nitroaromatic compounds by Thermotoga maritima hybrid peroxiredoxin-nitroreductase enzyme

Abstract : Explosives such as 2,4,6-trinitrotoluene (TNT) and related polynitroaromatics being toxic environmental pollutants, numerous efforts are directed towards their biodegradation. In this work, we studied the NADPH-dependent two-electron reduction of a number of nitroaromatic compounds by a peroxiredoxin-nitroreductase hybrid enzyme from Thermotoga maritima (Prx-NR). We have found that the peroxiredoxin and nitroreductase domains of Prx-NR function independently. The activity of Prx-NR towards nitroaromatics is not influenced by their particular structure and is characterized by a linear log k(cat)/K(m) dependence on their single-electron reduction potentials (E(7)(1)). The reduction of polynitroaromatic explosives N-nitramines such as tetryl (2,4,6-trinitrophenyl-N-methylnitramine) and pentryl (2,4,6-trinitrophenyl-N-nitroaminoethylnitrate) was accompanied by the formation of nitrite, which implies their reductive N-denitration, while p-dinitrobenzene is reduced to p-hydroxylaminonitrobenzene. Taken together, these data indicate that nitroreductase reactions of Prx-NR share common features with those of other bacterial oxygen-insensitive nitroreductases, e. g., Enterobacter cloacae nitroreductase. However, the activity of Prx-NR is 10-100 times lower than that of E. cloacae NR.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-02649470
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Friday, May 29, 2020 - 12:05:12 PM
Last modification on : Wednesday, September 8, 2021 - 4:02:08 PM

Identifiers

Collections

Citation

Nicolas Rouhier, Jean-Pierre Jacquot, Lina Miseviciene, Zilvinas Anusevicius, Jonas Sarlauskas, et al.. Two-electron reduction of nitroaromatic compounds by Thermotoga maritima hybrid peroxiredoxin-nitroreductase enzyme. Chemija, 2011, 22 (2), pp.143 - 148. ⟨hal-02649470⟩

Share

Metrics

Record views

10