Skip to Main content Skip to Navigation
Journal articles

Coloured Semi-reflective Thin Films for Biomass-hydrolyzing Enzyme Detection

Abstract : A new enzymatic activity detection assay based on colour change of the semi-reflective films is presented. The method is based on the preparation of multilayered thin films of controlled thickness obtained by sequential deposition of cellulose nanocrystals and xyloglucan. The hydrolysis of the films leads to a decrease in layer thickness that enables to detect enzyme activity, to the naked eye, from the resulting colour changes in a span of few minutes. The method allows direct, fast, highly sensitive, and easy-to-use characterization of enzymatic activities.
Complete list of metadata

https://hal.inrae.fr/hal-02652638
Contributor : Migration Prodinra <>
Submitted on : Friday, May 29, 2020 - 7:52:19 PM
Last modification on : Thursday, May 20, 2021 - 12:02:02 PM

Links full text

Identifiers

Collections

INRAE | INRA | CEA | CNRS

Citation

Carole Cerclier, Aurélie Lack, Céline Moreau, Fabrice Cousin, Nadège Beury, et al.. Coloured Semi-reflective Thin Films for Biomass-hydrolyzing Enzyme Detection. Advanced Materials, Wiley-VCH Verlag, 2011, 23 (33), pp.3791 - +. ⟨10.1002/adma.201101971⟩. ⟨hal-02652638⟩

Share

Metrics

Record views

22