Skip to Main content Skip to Navigation
New interface
Journal articles

Seasonal photoperiodism regulates the expression of cuticular and signalling protein genes in the pea aphid

Abstract : Seasonal photoperiodism in aphids is responsible for the spectacular switch from asexual to sexual reproduction. However, little is known on the molecular and physiological mechanisms involved in reproductive mode shift through the action of day length. Earlier works showed that aphid head, but not eyes, directly perceives the photoperiodic signal through the cuticle. In order to identify genes regulating the photoperiodic response, a 3321 cDNA microarray developed for the pea aphid, Acyrthosiphon pisum was used to compare RNA populations extracted from heads of short- and long-day reared aphids. Microarray analyses revealed that 59 different transcripts were significantly regulated, among which a majority encoded cuticular proteins and several encoded proteins involved in cellular signalling or signal transduction. These results were confirmed by quantitative RT-PCR experiments on two cuticular and three signalling protein genes. Complementary experiments eliminated moulting and circadian rhythms as putative confounding effects. Quantitative RT-PCR performed at additional developmental stages demonstrated the regulation of expression of cuticular and signalling protein genes during the whole process of photoperiod shortening. This suggests that photoperiodic changes could affect cuticle structure and cell to cell communication in the head of aphids in relation with the switch of reproductive modes
Keywords : ADNc
Document type :
Journal articles
Complete list of metadata
Contributor : Migration ProdInra Connect in order to contact the contributor
Submitted on : Friday, May 29, 2020 - 11:31:34 PM
Last modification on : Wednesday, April 6, 2022 - 4:08:07 PM

Links full text




Gaël Le Trionnaire, Stéphanie Jaubert, B. Sabater-Munoz, A. Benedetto, Joel Bonhomme, et al.. Seasonal photoperiodism regulates the expression of cuticular and signalling protein genes in the pea aphid. Insect Biochemistry and Molecular Biology, 2007, 37 (10), pp.1094-1102. ⟨10.1016/j.ibmb.2007.06.008⟩. ⟨hal-02656485⟩



Record views